Composite

Part:BBa_K2950000:Design

Designed by: Wanji Li   Group: iGEM19_BNDS_China   (2019-10-17)


pYES-GAL1 promoter-OKS-TDH1 terminator-TEF1 promoter-ZhuI-CYC1 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1769
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon-optimization


Source

We obtained the DNA sequence of GAL1 promoter, OKS, TEF1 promoter, ZhuI, and CYC1 terminator from literature and then got these several basic parts through the gene synthesis service provided by Beijing Genomics institution. After Gibson Assembly, all basic parts are connected together to form this composite part.

References