![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2950000:Design
Designed by: Wanji Li Group: iGEM19_BNDS_China (2019-10-17)
pYES-GAL1 promoter-OKS-TDH1 terminator-TEF1 promoter-ZhuI-CYC1 terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1769
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon-optimization
Source
We obtained the DNA sequence of GAL1 promoter, OKS, TEF1 promoter, ZhuI, and CYC1 terminator from literature and then got these several basic parts through the gene synthesis service provided by Beijing Genomics institution. After Gibson Assembly, all basic parts are connected together to form this composite part.